Fig. 2. HOTAIR knockdown increases the TET1 expression in HeLa cells. HeLa cells were transiently transfected with DsiCTRL or DsiHOTAIR and the levels of HOTAIR (A), mRNA (B), protein (C) and promoter methylation (D) of TET1 were determined 48 hours later by RT-qPCR, western blot and MSP, respectively. Three independent experiments were performed. The bars represent the mean ± standard deviation from at least three independent experiments. *p˂0.05.